产品货号：
Z33107
中文名称：
Alpha Tubulin/TUBA1A抗体
英文名称：
Anti-Alpha Tubulin/TUBA1A Antibody (Clone#TU-16)
产品规格：
0.1mg(C100)
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-Alpha Tubulin/TUBA1A Antibody (Clone#TU-16) |
| 指标别名 | alpha tubulin;TUBA1A;TUBA3 |
| 克隆性 | Monoclonal |
| 检验物种 | mouse,rat,pig,nicotiana,human,dog |
| 应用范围 | IP,WB,IHC,ICC,ELISA |
| 基因名称 | TUBA1 |
| 克隆号 | TU-16 |
| 抗体来源 | Mouse |
| 抗体类型 | IgM |
| 免疫原 | Porcine brain microtubule protein MTP-1.The antibody TU-16 reacts with alpha-tubulin of all tested species,under denaturing and non-denaturing conditions. |
| 计算分子量 | 50kDa |
| 成分 | Tris buffered saline (TBS),pH8.0,15mM sodium azide |
| 纯化方式 | Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods). |
| 浓度 | 1mg/mL |
| 产品形态 | 溶液 |
| 保存条件 | Store at 2～8℃.Do not freeze. |
| 背景资料 | The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs).The microtubules consist of 13 protofilaments and have an outer diameter 25nm.Microtubules have their intrinsic polarity;highly dynamic plus ends and less dynamic minus ends.Microtubules are required for vital processes in eukaryotic cells including mitosis,meiosis,maintenance of cell shape and intracellular transport.Microtubules are also necessary for movement of cells by means of flagella and cilia.In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs).The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule.In vivo,microtubule dynamics vary considerably.Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening每;this phenomenon is called dynamic instability of microtubules.On a practical level,microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg.GMPPCP) or more commonly by anti-cancer drugs such as Taxol.Taxol stabilizes microtubules at room temperature for many hours.Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains.The alpha-tubulin (relative molecular weight around 50kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules.In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes).Expressed tubulin genes are named tubulin isotypes.Some of the tubulin isotypes are expressed ubiquitously,while some have more restricted tissue expression.Alpha-tubulin is also subject of numerous post-translational modifications.Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms.Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain. |
| Uniprot ID | Q71U36 |
| 基因名全称 | Tubulin alpha 1 |
| 蛋白名全称 | Tubulin alpha-1A chain |
| 推荐稀释比 | Immunohistochemistry (paraffin sections):10μg/mL. Immunoprecipitation:Reducing conditions. Western blotting:1～2μg/mL.This antibody can be used for Western blotting,but its alternative TU-02 (11-447-C100) gives better signal in this application. |
相关搜索：Alpha Tubulin/TUBA1A抗体，Alpha Tubulin抗体，TUBA1A单抗，Anti-Alpha Tubulin/TUBA1A Antibody (Clone#TU-16)